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Cellular e Molecular Oncology Laboratory
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  • Research Team
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    • Primary Cell culture
    • Molecular biology and biochemistry
    • Next Generation Sequencing (NGS)
    • Bacterial culture
    • Flow cytometry
    • Confocal microscope and optical imaging
    • Histology and laboratory cleaning equipments
    • Animal house and small animal imaging
    • Cryopreservation facility
    • Metabolomic
    • Spatial Transcriptomic
    • Additional resources (offices and meeting room)
  • SOP
    • Cell Culture
      • Cell culture room procedures
      • New stem cell medium and digestive and organoid protocol
      • Isolation of adipose cells
      • Transfection CSCs fugene
      • Primary normal colon tissue culture
      • Organoids from primary cells
      • Generation of organoids from mouse small intestine
    • Molecular Biology and biochemistry
      • DNA extraction from cells
      • RNA extraction from cells
      • Protocol Fixation and inclusion in paraffin
      • Viral RNA isolation
      • Extraction RNA Trizol
      • Cytofluorimetry Protocol
      • RPPA
      • Immunofluorescence on coverslips
      • Immunostaining
      • Mallory-Azan
      • Hematoxylin Eosin
      • Orange Acridine
      • Cito included in paraffin
      • Tunel
      • Western Blot
      • Trasfection wa09 fugene
    • Animal house
      • PCR Rodent Infectious Agent (PRIA)
      • Extermination of the area (Animal House)
      • Sanitizing of area (Animal House)
      • Microbiological environmental control (animal house)
      • In Vivo Experiment
    • General
      • Cryopreservation of pieces anatomical OCT
      • Freezing of tissues for DNA, RNA and protein extraction
      • Freezing and thawing
      • Paraformaldehyde
      • Operating procedures for access to reagents and use of chemicals
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In Vivo Experiment

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Tags

50 anni AircAircAwardBreast CancerCancer Stem CellsColon CancerFondamentaleIL-4ImmunotherapyJPMobesitàOrganoidsPassenger MutationsStaminalistassiTodarotumoreUnipa

Contacts

Cellular and Molecular Oncology Laboratory

Via Del Vespro, 131 – 90127 Palermo

Mail: laboratoriostassi@gmail.com

Phone: +39 0916554083

Location

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