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Primary Cell culture
Molecular biology and biochemistry
Next Generation Sequencing (NGS)
Bacterial culture
Flow cytometry
Confocal microscope and optical imaging
Histology and laboratory cleaning equipments
Animal house and small animal imaging
Additional resources (offices and meeting room)
Cryopreservation facility
SOP
Cell Culture
Cell culture room procedures
New stem cell medium and digestive and organoid protocol
Isolation of adipose cells
Transfection CSCs fugene
Primary normal colon tissue culture
Organoids from primary cells
Generation of organoids from mouse small intestine
Molecular Biology and biochemistry
DNA extraction from cells
RNA extraction from cells
Protocol Fixation and inclusion in paraffin
Viral RNA isolation
Extraction RNA Trizol
Cytofluorimetry Protocol
RPPA
Immunofluorescence on coverslips
Immunostaining
Mallory-Azan
Hematoxylin Eosin
Orange Acridine
Cito included in paraffin
Tunel
Western Blot
Trasfection wa09 fugene
Animal house
PCR Rodent Infectious Agent (PRIA)
Extermination of the area (Animal House)
Sanitizing of area (Animal House)
Microbiological environmental control (animal house)
In Vivo Experiment
General
Cryopreservation of pieces anatomical OCT
Freezing of tissues for DNA, RNA and protein extraction
Freezing and thawing
Paraformaldehyde
Operating procedures for access to reagents and use of chemicals
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3.11.2016 Airc – Steri
Alternating training 2017/18
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